Poly(β-hydroxybutyrate) Production using Glycerol as a Carbon Source
Keywords:
Bacillus megaterium, modeling, poly(b-hydroxybutyrate).Abstract
Abstract
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In this work, we carry out a design and a preliminary dimensioning of a poly(b-hydroxybutyrate) (PHB) plant, based on microorganisms and using glycerol as raw material. The process comprises three main sectors: a) raw material purification, where the glycerol stream (coming from a biodiesel production plant) is purified by eliminating methanol; b) fermentation, where the growth of the microorganisms (Bacillus megaterium) takes place and the production of PHB occurs. In this point, a model of the specific growth rate for B. megaterium is implemented within a control vector parameterization approach; c) biopolymer separation, which includes the extraction process, where cellular walls are exposed to lyses and a purified product, is obtained.
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Key words:
Bacillus megaterium; modeling; poly(b-hydroxybutyrate).
References
Referencias
Aspen Plus version 7.2, Aspen Technology, Inc., Cambridge, Massachusetts, USA, 2010.
Cámara argentina de recursos renovables. Renovables. Recuperado de : http://www.cader.org.ar/informes-y-estudios/ evolucion-del-mercado-de-biocombustibles-en -la-argentina.htm [citado el 29 de Marzo de 2012].
López Jiménez, J. A (2011) Biopolímeros de interés industrial. Síntesis y caracterización de polihidroxibutirato (PHB). Bahia Blanca, Argentina. Universidad Nacional del Sur.
López, J. A., Bucalá, V. & Villar, M. A (2010) Application of Dynamic Optimization Techniques for Poly(Beta-hydroxybutyrate) Production in a Fed-Batch Bioreactor. Industrial & engineering chemistry research, 49, 1762-1769.
López, J. A., Naranjo J. M., Higuita J. C., Cubitto M. A., Cardona C. A. & Villar M. A (2012) Biosynthesis of PHB from a Nex Isolated Bacillus megaterium Strain: Outlook on future developments with endospore forming bacteria. Biotechnology and bioprocess engineering, 17, 250-258.
Posada, J. A., Cardona, C. A. & Rincón, L. E.(2010) Sustai-nable biodiesel production from palm using in situ produced glycerol and biomass for raw bioethanol. 32nd Symposium on biotechnology for fuels and chemicals. Clearwater Beach, Estados Unidos, Abril.
Posada, J. A., Naranjo, J. M., Lopéz, J. A., Higuita, J. C. & Cardona, C. A (2011) Design and analysis of PHB production processes from crude glycerol, Process biochemistry, 46, 310-317.
Ramos, F. D., Estrada, V., Diaz, M. S. & Villar, M. A (2013) Optimización de la producción de Poli(ï¢-hidroxi-butirato) a partir de Glicerol. 13º Simposio argentino de informática. Córdoba, Argentina, Septiembre
Renewables Global Status Report, 2013 Update, p. 30. Recuperado de : http://www.ren21.net/Portals/0/documents/Resources/GSR/2013/GSR2013_lowres.pdf
Ulrich, G. D. & Vasudevan, P. T. (2004) Short-Cut Equipment Design. En: Chemical Engineering Process Design and Economics: A Practical Guide (Ulrich y Vasudevan Eds.). McGraw-Hill, Durham, 89-324.