Inhibitory activity of acetylcholinesterase by 18 extracts Guatemala native plant species used in the treatment of nervous disorders
Keywords:
Lantana camara, Tagetes lucida, Valeriana prionophylla, Wigandia urens var. caracasana, Lippia graveolens, Chaptalia mutansAbstract
Abstract
Neurodegenerative syndromes generate memory and behavior deterioration, usually in the elderly.The most common is Alzheimer´s disease, which is associated with decreased levels of acetylcholine(ACh) after an increase of acetylcholinesterase (AChE). Worldwide studies have described somesecondary metabolites from plants that might inhibit the enzyme activity or retard neuronal deterioration.With the aim of finding inhibitors of AChE, activity was evaluated in extracts (dichloromethane andmethanol) of 18 medicinal and aromatic plants used in Guatemala for nervous or memory disorders(Brugmansia candida, Cassia reticulata, Chaptalia nutans, Chiranthodendron pentadactylon, Dorsteniacontrajerva, Erythrina berteroana, Lantana camara, Lippia graveolens, Petiveria alliacea, Phlebodiumpseudoaureum, Pimenta dioica, Salvia microphylla, Solanum nigrescens, Tagetes lucida, Ternstroemiatepezapote, Valeriana prionophylla, Vernonia deppeana and Wigandia urens var. caracasana). AChEinhibition was assessed qualitatively by two bioautographic TLC assays and quantitatively by akinetic microcolorimetric evaluation. The results demonstrated activity by bioautography in all speciesat screening concentration (5 μl), but when quantified by microcolorimetry no significant activity(>50%) was demonstrated at 1 mg/mL. Three of the species with shown activity (L. camara, T. lucidaand V. prionophylla) were partitioned to see if the liquid:liquid partions presented more activity, butthis did not increase the activity. Intermediate activity was also found in extracts from C. nutans, D.contrajerva var. houstoni, L. graveolens and W. urens var. caracasana. Studies will continue regardingthe antioxidant activity in order to determine whether there might be some potential use.
Keywords:
Lantana camara, Tagetes lucida, Valeriana prionophylla, Wigandia urens var. caracasana,Lippia graveolens, Chaptalia nutans.
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