Detection of blaKPC and blaNDM carbapenemases genes in Klebsiella pneumoniae isolates from San Juan de Dios General Hospital in Guatemala City, Guatemala
Keywords:
carbapenemases, blaNDM gene, blaKPC gene, Klebsiella pneumoniae.Abstract
Resistance to antibiotics is one of the most important public health problems worldwide. Enterobacteriaceae, producers of carbapenemases, pose the greatest threat. Carbapenemases are powerful enzymes that inactivate carbapenem antibiotics and generally all beta-lactam antibiotics. The consequences that result from treating infections caused by these bacteria are important because carbapenems are the latest options available for multidrug-resistant bacteria. This research aimed to determine by the means of endpoint polymerase chain reaction (PCR), the presence of blaKPC (Klebsiella pneumoniae carbapenemase) and blaNDM (New Delhi metallolactamase) carbapenemases genes in Klebsiella pneumoniae isolates from San Juan de Dios General Hospital located in Guatemala City; to characterize the sample type and determine the area of the hospital where such bacteria were isolated. 54 Klebsiella pneumoniae isolates resistant to carbapenems (imipenem and / or meropenem), were analyzed. From these, 49 (91 %) were detected as blaNDM gene carriers. These bacteria were more frequently isolated in blood (37%) and urine (14%) samples. In this research, 53% of the isolates were obtained from patients hospitalized in intensive care units. This study demonstrates that Klebsiella pneumoniae harboring blaNDM gene has spread within San Juan de Dios General Hospital, since the first reported case 5 years ago, risking the life of patients, especially of those hospitalized in intensive care units.
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