Assessment of air pollution by microscopic fungi in the Herbarium of the UNiversity of San Carlos of Guatemala, Index section Seminum and macrofungi of Guatemala Biology Herbarium
Keywords:
air quality, microscopic fungi, biocolector, herbarium, air samplingAbstract
Abstract
The indoor and outdoor quality air was determined at the University of San Carlos of GuatemalaHerbarium (USCG Herbarium), the Index seminum and macrofungi section of Biology of GuatemalaHerbarium (BIGU Herbarium). It was used a volumetric impactation technic through a biocolectorin order to collect microscopic fungi. Sampling was carried out from October 2011 through March2012. Further identification was performed through microscopic observation using lactophenol bluestaining and also using API 20C AUX was for yeast identification. Results indicate that in USCGHerbarium environments, the highest concentration in the outdoor 3580 UFC/m3 and 300 UFC/m3for the indoor, Index seminum 1860 UFC/m3 for the outdoor and 2300 UFC/m3 for the indoor, tomicrofungi section of BIGU Herbarium the highest concentration was 2790 UFC/m3 for the outdoorand 1630 UFC/m3 for the indoor.During the months in wich the sampling was carried out, it was observed that the predominant fungiin both environments and in all sampled areas were Penicillium sp, Cladosporium sp y Aspergillussp. It was possible to isolate fungal genera of great importance for its cellulolytic and phytopathogenicaction, such as Aspergillus sp., Penicillium sp., Fusarium sp. y Paecilomyces sp. The isolation ofthe fungal genera made it possible to create a strain library applying the mineral oil conservationtechnique.It was created a guide containing information about cleaning procedures according to biosafetyinfrastructure and raw materials for each establishment.
Keywords:
Air quality, microscopic fungi, biocolector, herbarium, air sampling.
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FACULTAD DE CIENCIAS QUÍMICAS Y FARMACIA
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